cleaved n terminal gsdmd antibody  (MedChemExpress)

Journal: Experimental and Therapeutic Medicine

Article Title: Maresin 1 activates autophagy through SIRT1/PPAR-γ signaling to mitigate high glucose-induced pyroptosis in human retinal pigment epithelial cells

doi: 10.3892/etm.2026.13134

Figure Lengend Snippet: MaR1 ameliorates HG-induced pyroptosis in ARPE-19 cells. (A) Effect of MaR1 treatment at varying concentrations on the fluorescence intensity of GSDMD-N in HG-induced ARPE-19 cells. Scale bar, 50 µm. (B) Effect of MaR1 treatment at varying concentrations on the expression of pyroptosis-related proteins (caspase-1, GSDMD, GSDMD-N, cleaved caspase-1, NLRP3, ASC and IL-18) in HG-induced ARPE-19 cells. * P<0.05, ** P<0.01 and *** P<0.001. MaR1, maresin 1; HG, high glucose; NG, normal glucose; GSDMD, gasdermin D; GSDMD-N, GSDMD N-terminal; NLRP3, NLR family pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing a CARD.

Article Snippet: Subsequently, the cells were blocked with 1% BSA solution (cat. no. ST023; Beyotime Biotechnology) at room temperature for 1 h, followed by overnight incubation at 4 ̊C with diluted anti-GSDMD N-terminal (GSDMD-N) primary antibody (1:100; cat. no. ER1901-37; HUABIO).

Techniques: Fluorescence, Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Maresin 1 activates autophagy through SIRT1/PPAR-γ signaling to mitigate high glucose-induced pyroptosis in human retinal pigment epithelial cells

doi: 10.3892/etm.2026.13134

Figure Lengend Snippet: MaR1 mediates autophagy through SIRT1/peroxisome proliferator-activated receptor-γ signaling to ameliorate HG-induced pyroptosis in ARPE-19 cells. (A) SIRT1 inhibitor EX527 and autophagy inhibitor 3-MA enhanced the fluorescence intensity of GSDMD-N in ARPE-19 cells treated with HG and MaR1. Scale bar, 50 µm. (B) EX527 and 3-MA upregulated the expression of GSDMD-N, cleaved caspase-1, NLRP3, ASC and IL-18 in ARPE-19 cells treated with HG and MaR1. * P<0.05, ** P<0.01 and *** P<0.001. MaR1, maresin 1; HG, high glucose; NG, normal glucose; SIRT1, sirtuin 1; GSDMD, gasdermin D; GSDMD-N, GSDMD N-terminal; NLRP3, NLR family pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing a CARD; 3-MA, 3-methyladenine.

Article Snippet: Subsequently, the cells were blocked with 1% BSA solution (cat. no. ST023; Beyotime Biotechnology) at room temperature for 1 h, followed by overnight incubation at 4 ̊C with diluted anti-GSDMD N-terminal (GSDMD-N) primary antibody (1:100; cat. no. ER1901-37; HUABIO).

Techniques: Fluorescence, Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Maresin 1 activates autophagy through SIRT1/PPAR-γ signaling to mitigate high glucose-induced pyroptosis in human retinal pigment epithelial cells

doi: 10.3892/etm.2026.13134

Figure Lengend Snippet: Schematic illustration of the protective mechanism of MaR1 against HG-induced pyroptosis in ARPE-19 cells. High glucose stimulation triggers the overproduction of ROS, leading to the activation of the NLRP3 inflammasome complex (comprising NLRP3, ASC and Caspase-1). This activation results in the cleavage of GSDMD, which forms membrane pores (GSDMD-N) and promotes the release of pro-inflammatory cytokines, ultimately inducing pyroptosis. However, MaR1 treatment effectively upregulates SIRT1 expression, which in turn enhances PPAR-γ levels and restores autophagic flux. The restored autophagy facilitates the clearance of damaged mitochondria and ROS, thereby inhibiting the NLRP3/GSDMD signaling pathway and protecting ARPE-19 cells from HG-induced inflammatory damage. SIRT1, sirtuin 1; PPAR-γ, peroxisome proliferator-activated receptor-γ; ROS, reactive oxygen species; GSDMD-N, gasdermin D N-terminal; NLRP3, NLR family pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing a CARD.

Article Snippet: Subsequently, the cells were blocked with 1% BSA solution (cat. no. ST023; Beyotime Biotechnology) at room temperature for 1 h, followed by overnight incubation at 4 ̊C with diluted anti-GSDMD N-terminal (GSDMD-N) primary antibody (1:100; cat. no. ER1901-37; HUABIO).

Techniques: Activation Assay, Membrane, Expressing

Journal: mSystems

Article Title: Taurine attenuates Listeria monocytogenes -induced inflammation and pyroptosis in mouse model by regulating MAPK and NLRP3/caspase-1/GSDMD pathways

doi: 10.1128/msystems.01043-25

Figure Lengend Snippet: Proposed mechanism of taurine against Lm infection. Taurine targets Lm virulence and host pyroptosis. Taurine inhibits bacterial pathogenesis while balancing immune responses: activating MAPK signaling pathway for T-cell immunity and suppressing NLRP3/GSDMD-mediated pyroptosis.

Article Snippet: Equal amounts of protein (30 μg per lane) were separated by 10% SDS-PAGE gel electrophoresis and transferred to PVDF membrane with 5% skimmed milk closed for 1 h. The target proteins on the membranes were probed with the specific primary antibodies as follows: rabbit anti-JNK1 + JNK2 + JNK3 polyclonal antibody (Bioss, bs-2592R), rabbit anti-phospho-JNK1/2/3 (T183 + T183 + T221) monoclonal antibody (Bioss, bsm-52462R), rabbit anti-ERK1/2 monoclonal antibody (Bioss, bsm-52259R), rabbit anti-phospho-ERK1/2 (Thr202 + Thr204) polyclonal antibody (Bioss, bs-3016R), p38 MAPK rabbit mAb (CST, 8690), phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb (CST, 4511), IL-1β pAb (Bioss, bs-812R), caspase 1/p20/p10 polyclonal antibody (Proteintech, 22915-1-AP), total and cleaved N-terminal GSDMD antibody (Abmart, P79887R), and NLRP3 monoclonal antibody (Proteintech, 68102-1-Ig).

Techniques: Infection

Journal: Cells

Article Title: Machine Learning-Driven Multi-Omics Analysis Identifies CHP2 as a Key PANoptosis-Related Dual-Function Biomarker in Colorectal Cancer

doi: 10.3390/cells15050430

Figure Lengend Snippet: Experimental validation of CHP2 as a predictive biomarker for drug sensitivity and a driver of PANoptosis. ( A – C ) IC 50 growth inhibition curves of HCT116 and SW480 cells treated with first-line chemotherapeutic agents: ( A ) 5-fluorouracil (5-FU), ( B ) Oxaliplatin, and ( C ) Irinotecan. ( D , E ) IC50 curves for targeted agents: ( D ) Ribociclib and ( E ) Lapatinib. ( F ) Western blot analysis of key PANoptosis markers, including p-MLKL (necroptosis), N-GSDMD (pyroptosis), and Cleaved-Caspase 3 (apoptosis), in Control, CHP2-OV, and CHP2-OV-siRNA-3 groups. ( G – J ) Quantitative analysis of protein expression levels normalized to β-actin. Data are presented as mean ±SD from three independent experiments. * p < 0.05, ** p < 0.01.

Article Snippet: After the protein concentration was determined with a BCA protein assay kit (Beyotime, P0010), identical amounts of proteins were subjected to SDS-PAGE and transferred onto PVDF membranes, which were subsequently incubated with primary antibodies against CHP2 (Boster, Wuhan, China, A08478-2, 1:1000), p-MLKL (MCE, HY86069, 1:1000), GSDMD (N-terminal) (MCE, HY- P85810 , 1:1000), Cleaved Caspase-3 (HY- P86370 ) and β-actin (Servicebio, ZB15001-HRP, 1:2000).

Techniques: Biomarker Discovery, Inhibition, Western Blot, Control, Expressing

Journal: BMC Immunology

Article Title: Diagnostic and prognostic value of deregulated miR-6822-3p in patients with severe pneumonia

doi: 10.1186/s12865-026-00801-3

Figure Lengend Snippet: miR-6822-3p promotes pyroptosis in THP-1 cells. A Expression levels of miR-6822-3p in THP-1 cells. B Western blot (WB) analysis of pyroptosis-related proteins in THP-1 cells. C The quantification of NLRP3 by WB. D The quantification of cleaved caspase-1 by WB. E The quantification of cleaved GSDMD by WB. F IL-1β levels in THP-1 cell supernatants. G IL-18 levels in THP-1 cell supernatants. H Changes in VE-cadherin mRNA expression in HLMVEC cells

Article Snippet: The primary antibodies used in the experiment were NLRP3 (Beyotime, AF2155), cleaved caspase-1 (MCE, HY- P80622 ), cleaved N-terminal GSDMD (MCE, HY- P86158 ), and β-actin (Beyotime, AF5003).

Techniques: Expressing, Western Blot